International journal of molecular sciences, 2021-10, Vol.22 (21), p.11724
2021
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- Autor(en) / Beteiligte
- Titel
- Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
- Ist Teil von
- International journal of molecular sciences, 2021-10, Vol.22 (21), p.11724
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic carcinogenic compounds and non-carcinogenic compounds on the expression and functionality of connexin-based channels, both gap junctions and connexin hemichannels, were investigated in human hepatoma HepaRG cell cultures. Expression of connexin26, connexin32, and connexin43 was evaluated by means of real-time reverse transcription quantitative polymerase chain reaction analysis, immunoblot analysis and in situ immunostaining. Gap junction functionality was assessed via a scrape loading/dye transfer assay. Opening of connexin hemichannels was monitored by measuring extracellular release of adenosine triphosphate. It was found that both genotoxic and non-genotoxic carcinogenic compounds negatively affect connexin32 expression. However, no specific effects related to chemical type were observed at gap junction or connexin hemichannel functionality level.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms222111724Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3b4681c2e07e4da8bba08f8e6243ce5a
- Format
- –
- Schlagworte
- Adenosine triphosphate, ATP, carcinogen, Carcinogens, Carcinogens - toxicity, Carcinoma, Hepatocellular - chemically induced, Carcinoma, Hepatocellular - genetics, Carcinoma, Hepatocellular - metabolism, Cell Line, Tumor, Channel gating, Channels, Chemicals, connexin, Connexin 26 - genetics, Connexin 26 - metabolism, Connexin 32, Connexin 43, Connexin 43 - genetics, Connexin 43 - metabolism, Connexins - genetics, Connexins - metabolism, gap junction, Gap Junction beta-1 Protein, Gap junctions, Gap Junctions - drug effects, Gap Junctions - genetics, Gap Junctions - metabolism, Gene expression, Gene Expression Regulation, Neoplastic - drug effects, genotoxic, Genotoxicity, hemichannel, Hepatoma, Humans, Liver diseases, Liver Neoplasms - chemically induced, Liver Neoplasms - genetics, Liver Neoplasms - metabolism, Localization, non-genotoxic, Polymerase chain reaction, Protein expression, Proteins, Reverse transcription