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WRKY Transcription Factors Associated With NPR1 -Mediated Acquired Resistance in Barley Are Potential Resources to Improve Wheat Resistance to Puccinia triticina
Ist Teil von
Frontiers in plant science, 2018-10, Vol.9, p.1486-1486
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2018
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Systemic acquired resistance (SAR) in
is established beyond the initial pathogenic infection or is directly induced by treatment with salicylic acid or its functional analogs (SA/INA/BTH). NPR1 protein and WRKY transcription factors are considered the master regulators of SAR. Our previous study showed that
homologs in wheat (
L.) and barley (
L.) regulated the expression of genes encoding pathogenesis-related (PR) proteins during acquired resistance (AR) triggered by
pv.
DC3000. In the present examination, AR induced by
DC3000 was also found to effectively improve wheat resistance to
(
). However, with more complex genomes, genes associated with this SAR-like response in wheat and barley are largely unknown and no specific
has been reported to be involved in this biological process. In our subsequent analysis, barley transgenic line overexpressing wheat
(wNPR1-OE) showed enhanced resistance to
isolate Guy11, whereas AR to Guy11 was suppressed in a barley transgenic line with knocked-down barley
(HvNPR1-Kd). We performed RNA-seq to reveal the genes that were differentially expressed among these transgenic lines and the wild-type barley plants during the AR. Several
and BTH-induced (
) genes were designated as downstream genes of
. The expression of few
was significantly associated with
expression during the AR events. The transient expression of three
genes, including
,
, and
, in wheat leaves by
-mediated infiltration enhanced the resistance to
. In conclusion, a profile of genes associated with
-mediated AR in barley was drafted and
discovered in the current study showed a substantial potential for improving wheat resistance to
.