Details

Autor(en) / Beteiligte

• Guo, Dongxue
• Lin, Shudai
• Wang, Xiaotong
• Jiao, Zhenhai
• Li, Guo
• An, Lilong
• Zhang, Zihao
• Zhang, Li

Titel

Establishment and Characterization of a Chicken Myoblast Cell Line

Ist Teil von

• International journal of molecular sciences, 2024-08, Vol.25 (15), p.8340

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Skeletal muscle, which is predominantly constituted by multinucleated muscle fibers, plays a pivotal role in sustaining bodily movements and energy metabolism. Myoblasts, which serve as precursor cells for differentiation and fusion into muscle fibers, are of critical importance in the exploration of the functional genes associated with embryonic muscle development. However, the in vitro proliferation of primary myoblasts is inherently constrained. In this study, we achieved a significant breakthrough by successfully establishing a chicken myoblast cell line through the introduction of the exogenous chicken telomerase reverse transcriptase ( ) gene, followed by rigorous G418-mediated pressure screening. This newly developed cell line, which was designated as -myoblasts, closely resembled primary myoblasts in terms of morphology and exhibited remarkable stability in culture for at least 20 generations of population doublings without undergoing malignant transformation. In addition, we conducted an exhaustive analysis that encompassed cellular proliferation, differentiation, and transfection characteristics. Our findings revealed that the -myoblasts had the ability to proliferate, differentiate, and transfect after multiple rounds of population doublings. This achievement not only furnished a valuable source of homogeneous avian cell material for investigating embryonic muscle development, but also provided valuable insights and methodologies for establishing primary cell lines.