Nature communications, 2017-09, Vol.8 (1), p.438-14, Article 438
2017
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- Autor(en) / Beteiligte
- Titel
- pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification
- Ist Teil von
- Nature communications, 2017-09, Vol.8 (1), p.438-14, Article 438
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The precise and large-scale identification of intact glycopeptides is a critical step in glycoproteomics. Owing to the complexity of glycosylation, the current overall throughput, data quality and accessibility of intact glycopeptide identification lack behind those in routine proteomic analyses. Here, we propose a workflow for the precise high-throughput identification of intact N-glycopeptides at the proteome scale using stepped-energy fragmentation and a dedicated search engine. pGlyco 2.0 conducts comprehensive quality control including false discovery rate evaluation at all three levels of matches to glycans, peptides and glycopeptides, improving the current level of accuracy of intact glycopeptide identification. The N-glycoproteome of samples metabolically labeled with N/ C were analyzed quantitatively and utilized to validate the glycopeptide identification, which could be used as a novel benchmark pipeline to compare different search engines. Finally, we report a large-scale glycoproteome dataset consisting of 10,009 distinct site-specific N-glycans on 1988 glycosylation sites from 955 glycoproteins in five mouse tissues.Protein glycosylation is a heterogeneous post-translational modification that generates greater proteomic diversity that is difficult to analyze. Here the authors describe pGlyco 2.0, a workflow for the precise one step identification of intact N-glycopeptides at the proteome scale.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-017-00535-2Titel-ID: cdi_doaj_primary_oai_doaj_org_article_396d0831a0fc493eb33a0808839d479d
- Format
- –
- Schlagworte
- Animals, Antibiotics, Carbon Isotopes, Glycopeptides, Glycopeptides - analysis, Glycopeptides - metabolism, Glycoproteins, Glycosylation, High-Throughput Screening Assays - methods, Humans, Male, Mass spectrometry, Mass spectroscopy, Methods, Mice, Inbred C57BL, Nitrogen Isotopes, Peptides, Pipelines, Polysaccharides - analysis, Polysaccharides - metabolism, Post-translation, Protein Processing, Post-Translational, Proteins, Proteomics - methods, Quality Control, Quantitative analysis, Scientific imaging, Search Engine, Search engines, Software, Tandem Mass Spectrometry - methods, Workflow