Details

Autor(en) / Beteiligte

• Ferrarese, Leiron
• Jouhanneau, Jean-Sébastien
• Remme, Michiel W.H.
• Kremkow, Jens
• Katona, Gergely
• Rózsa, Balázs
• Schreiber, Susanne
• Poulet, James F.A.

Titel

Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo

Ist Teil von

• Cell reports (Cambridge), 2018-09, Vol.24 (13), p.3455-3465.e5

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Excitatory synaptic input reaches the soma of a cortical excitatory pyramidal neuron via anatomically segregated apical and basal dendrites. In vivo, dendritic inputs are integrated during depolarized network activity, but how network activity affects apical and basal inputs is not understood. Using subcellular two-photon stimulation of Channelrhodopsin2-expressing layer 2/3 pyramidal neurons in somatosensory cortex, nucleus-specific thalamic optogenetic stimulation, and paired recordings, we show that slow, depolarized network activity amplifies small-amplitude synaptic inputs targeted to basal dendrites but reduces the amplitude of all inputs from apical dendrites and the cell soma. Intracellular pharmacology and mathematical modeling suggests that the amplification of weak basal inputs is mediated by postsynaptic voltage-gated channels. Thus, network activity dynamically reconfigures the relative somatic contribution of apical and basal inputs and could act to enhance the detectability of weak synaptic inputs. [Display omitted] •In vivo subcellular optogenetic stimulation of cortical layer 2/3 pyramidal neurons•Slow network activity amplifies small-amplitude basal dendritic inputs•Apical inputs are reduced during depolarized phases of slow network activity•Basal input amplification is mediated by postsynaptic voltage-gated channels Ferrarese et al. investigate the impact of network activity on synaptic integration in cortical L2/3 pyramidal neurons in vivo. They report a reduction of apical dendritic inputs but an amplification of small-amplitude basal inputs during depolarized phases of slow network activity. The amplification is dependent on postsynaptic voltage-gated channels.