Details

Autor(en) / Beteiligte

• Gonçalves da Costa Sousa, Mauricio
• Conceição de Almeida, Gabriela
• Martins Mota, Danilo César
• Andrade da Costa, Rosiane
• Dias, Simoni Campos
• Limberger, Samuel Nunes
• Ko, Frank
• Lin, Li Ting
• Haney, Evan F.
• Etayash, Hashem
• Baquir, Beverlie
• Trimble, Michael J.
• Shen, Ya
• Su, Zheng
• Haapasalo, Markus
• Pletzer, Daniel
• Chaves de Souza, Letícia
• Schuindt Teixeira, Gláucia
• Silva, Renato M.
• Hancock, Robert E.W.
• Franco, Octavio Luiz
• Berto Rezende, Taia Maria

Titel

Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Ist Teil von

• Bioactive materials, 2022-10, Vol.16, p.173-186

Ort / Verlag

China: Elsevier B.V

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry. Here, different scaffolds were tested for use during the pulp revascularization process, including poly(vinyl alcohol)-PVA hydrogels or resins, collagen hydrogels and poly(vinyl alcohol) PVA/Chitosan (PVA/CS) nanofibers. Based on time to degradation (21 days), nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002 (each at 50 mg/g). Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis, Staphylococcus aureus and a multispecies oral biofilm, besides anti-inflammatory activities. The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation, when compared to empty teeth fragments. Thus, we designed a strong antimicrobial, immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures. [Display omitted] -Nanofibers are stable scaffolds in culture media environment-IDR-1002 remained until nanofibers were degraded-Antibiofilm activity of the nanofibers was due to both ciprofloxacin and IDR-1002-Nanofibers were biocompatible and presented immunomodulatory activity in vitro-Nanofibers physically interacted with SCAPs in vitro and stimulated loose connective tissue formation in vivo