Details

Autor(en) / Beteiligte

• Marquina-Solis, Javier
• Feng, Likui
• Vandewyer, Elke
• Beets, Isabel
• Hawk, Josh
• Colón-Ramos, Daniel A.
• Yu, Jingfang
• Fox, Bennett W.
• Schroeder, Frank C.
• Bargmann, Cornelia I.

Titel

Antagonism between neuropeptides and monoamines in a distributed circuit for pathogen avoidance

Ist Teil von

• Cell reports (Cambridge), 2024-04, Vol.43 (4), p.114042-114042, Article 114042

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Pathogenic infection elicits behaviors that promote recovery and survival of the host. After exposure to the pathogenic bacterium Pseudomonas aeruginosa PA14, the nematode Caenorhabditis elegans modifies its sensory preferences to avoid the pathogen. Here, we identify antagonistic neuromodulators that shape this acquired avoidance behavior. Using an unbiased cell-directed neuropeptide screen, we show that AVK neurons upregulate and release RF/RYamide FLP-1 neuropeptides during infection to drive pathogen avoidance. Manipulations that increase or decrease AVK activity accelerate or delay pathogen avoidance, respectively, implicating AVK in the dynamics of avoidance behavior. FLP-1 neuropeptides drive pathogen avoidance through the G protein-coupled receptor DMSR-7, as well as other receptors. DMSR-7 in turn acts in multiple neurons, including tyraminergic/octopaminergic neurons that receive convergent avoidance signals from the cytokine DAF-7/transforming growth factor β. Neuromodulators shape pathogen avoidance through multiple mechanisms and targets, in agreement with the distributed neuromodulatory connectome of C. elegans. [Display omitted] •C. elegans avoids the pathogen P. aeruginosa after prolonged exposure•FLP-1 neuropeptides released by two AVK neurons drive pathogen avoidance•FLP-1 neuropeptides act through the G protein-coupled receptor DMSR-7•The peptidergic signaling pathway antagonizes tyraminergic/octopaminergic neurons Marquina-Solis et al. show that RF/RYamide FLP-1 neuropeptides promote an acquired pathogen avoidance behavior in C. elegans. FLP-1 neuropeptides regulate multiple targets in a distributed neuromodulatory circuit, including tyraminergic/octopaminergic neurons that inhibit pathogen avoidance. Pathogen exposure increases expression and release of flp-1 and decreases expression of a tyramine/octopamine biosynthetic gene.