Details

Autor(en) / Beteiligte

• Dukić, Josipa
• Košpić, Karla
• Kelava, Vanja
• Mavrić, Renata
• Nutrizio, Marinela
• Balen, Biljana
• Butorac, Ana
• Halil Öztop, Mecit
• Režek Jambrak, Anet

Titel

Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge

Ist Teil von

• Ultrasonics sonochemistry, 2023-10, Vol.99, p.106535-106535, Article 106535

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• •Waste utilization is in line with sustainable goals.•Sugar beet leaf, a waste from sugar industry, is a good source of RuBisCO enzyme.•US and HVED showed to be a good techniques for the extraction of Rubisco enzyme.•Higher yield of RuBisCO enzyme is achieved by US. Ultrasound (US) and high voltage electric discharge (HVED) with water as a green solvent represent promising novel non-thermal techniques for protein extraction from sugar beet (Beta vulgaris subsp. vulgaris var. altissima) leaves. Compared to HVED, US proved to be a better alternative method for total soluble protein extraction with the aim of obtaining high yield of ribulose-1,5-bisphosphate carboxylase-oxygenase enzyme (RuBisCO). Regardless of the solvent temperature, the highest protein yields were observed at 100% amplitude and 9 min treatment time (84.60 ± 3.98 mg/gd.m. with cold and 96.75 ± 4.30 mg/gd.m. with room temperature deionized water). US treatments at 75% amplitude and 9 min treatment time showed the highest abundance of RuBisCO obtained by immunoblotting assay. The highest protein yields recorded among HVED-treated samples were observed at a voltage of 20 kV and a treatment time of 3 min, disregarding the used gas (33.33 ± 1.06 mg/gd.m. with argon and 34.89 ± 1.59 mg/gd.m. with nitrogen as injected gas), while the highest abundance of the RuBisCO among HVED-treated samples was noticed at 25 kV voltage and 3 min treatment time. By optimizing the US and HVED parameters, it is possible to affect the solubility and improve the isolation of RuBisCO, which could then be purified and implemented into new or already existing functional products.