Malaria journal, 2020-07, Vol.19 (1), p.275-275, Article 275
- Rocha, Daniele
- de Melo, Gisely Cardoso
- Carneiro, José Marcelo Hipólito
- Ribeiro, Marisa
- Ribeiro, Sthefanie
- de Godoy, Daniela Tupy
- Costa, Elaine
- de Almeida, Anne Cristine Gomes
- de Andrade, Elisabete Ferreira
- Abrahim, Cláudia Maria de Moura
- Fraiji, Nelson Abrahim
- Ferreira, Antonio Gomes Pinto
- Monteiro, Wuelton Marcelo
- Brindeiro, Rodrigo
- Tanuri, Amilcar
- de Lacerda, Marcus Vinicius Guimarães
- Alvarez, Patrícia
2020
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- Autor(en) / Beteiligte
- Rocha, Daniele
- de Melo, Gisely Cardoso
- Carneiro, José Marcelo Hipólito
- Ribeiro, Marisa
- Ribeiro, Sthefanie
- de Godoy, Daniela Tupy
- Costa, Elaine
- de Almeida, Anne Cristine Gomes
- de Andrade, Elisabete Ferreira
- Abrahim, Cláudia Maria de Moura
- Fraiji, Nelson Abrahim
- Ferreira, Antonio Gomes Pinto
- Monteiro, Wuelton Marcelo
- Brindeiro, Rodrigo
- Tanuri, Amilcar
- de Lacerda, Marcus Vinicius Guimarães
- Alvarez, Patrícia
- Titel
- Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
- Ist Teil von
- Malaria journal, 2020-07, Vol.19 (1), p.275-275, Article 275
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Malaria can be transmitted by blood transfusion through donations collected from asymptomatic donors. Transfusion-transmitted malaria (TTM) poses a great risk to blood services worldwide. A good screening tool for Plasmodium spp. detection in blood banks must have a high sensitivity for prevention of TTM. However, in Brazilian blood banks, screening for malaria still relies on microscopy. In Brazil, screening for human immunodeficiency virus type 1 (HIV), RNA/DNA for hepatitis C (HCV) and hepatitis B (HBV) viruses is mandatory for every blood donation and uses nucleic acid amplification testing (NAT). The aim of this study was to evaluate the inclusion of an assay for malaria to identify Plasmodium sp. from total nucleic acid (TNA; DNA/RNA) by targeting the 18S rRNA gene of the parasite. Considering the limitations of microscopy and the wide availability of the Brazilian NAT platform in the screening of blood units for HIV, HCV, and HBV, a molecular diagnostic tool was validated for detection of Plasmodium sp. in blood banks; a pilot study showed that using this novel NAT assay could reduce the risk of TTM. The prototype HIV/HCV/HBV/malaria NAT assay was effective in detecting infected candidate donors and has good prospects to be applied in routine screening for preventing TTM.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1475-2875eISSN: 1475-2875DOI: 10.1186/s12936-020-03345-yTitel-ID: cdi_doaj_primary_oai_doaj_org_article_37a2bc98fa3a436dacc477b830813e34
- Format
- –
- Schlagworte
- Adolescent, Adult, Blood, Blood & organ donations, Blood Banks, Blood donors, Blood Transfusion, Brazil, Care and treatment, Deoxyribonucleic acid, Detection, Development and progression, Disease transmission, DNA, Epidemiological Monitoring, Female, Genetic testing, Health aspects, Hemovigilance, Hepatitis, Hepatitis B, Hepatitis B - diagnosis, Hepatitis C, Hepatitis C - diagnosis, HIV, HIV Infections - diagnosis, Human diseases, Human immunodeficiency virus, Humans, Infections, Malaria, Malaria - prevention & control, Malaria - transmission, Male, Mass Screening - instrumentation, Mass Screening - methods, Medical research, Methods, Microscopy, Middle Aged, Molecular diagnostic, Nucleic Acid Amplification Techniques - methods, Nucleic acids, Parasites, Pilot Projects, Plasma, Plasmodium, Plasmodium - genetics, Plasmodium - isolation & purification, Population Surveillance - methods, Prevention, Prototypes, Public health, Risk reduction, RNA, RNA viruses, RNA, Protozoan - analysis, RNA, Ribosomal, 18S - analysis, rRNA 18S, Sentinel surveillance, Transfusion, Transfusion-transmitted malaria, Tropical diseases, Vector-borne diseases, Viruses, Work stations, Young Adult