International journal of molecular sciences, 2019-07, Vol.20 (13), p.3303
2019
Details
- Autor(en) / Beteiligte
- Titel
- Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L
- Ist Teil von
- International journal of molecular sciences, 2019-07, Vol.20 (13), p.3303
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in is not well understood. Here, we report that the M-locus protein kinase ( ) , the functional homolog of in , controls SI in . We identified four paralogue genes in , including , , , and . Two transcripts of , and , were generated by alternative splicing. Tissue expression pattern analysis demonstrated that , especially , is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of in SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of in regulating the SI response of . We found that the expression of SI positive regulators S-locus receptor kinase ( ) and Arm-Repeat Containing 1 ( ) are suppressed in mutant, whereas the self-compatibility (SC) element Glyoxalase I ( ) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of in the SI of
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms20133303Titel-ID: cdi_doaj_primary_oai_doaj_org_article_36d37e49f2a0451f9b9891ed884787c2
- Format
- –
- Schlagworte
- Amino Acid Sequence, Base Sequence, Brassica napus, Brassica napus - enzymology, Brassica napus - genetics, Brassica napus - physiology, Cloning, CRISPR, CRISPR-Cas Systems - genetics, CRISPR/Cas9, Functional analysis, Gene Editing, Gene Expression Regulation, Plant, Gene frequency, Genes, Genomes, Haplotypes, Incompatibility, Kinases, Ligands, M locus protein kinase (MLPK), Mutation, Mutation - genetics, Organ Specificity - genetics, Phylogenetics, Phylogeny, Plant reproduction, Plants, Genetically Modified, Pollination, Protein Isoforms - chemistry, Protein Isoforms - genetics, Protein Isoforms - metabolism, Protein kinase, Protein Kinase C - chemistry, Protein Kinase C - genetics, Protein Kinase C - metabolism, Proteins, Rape plants, RNA Interference, RNA, Messenger - genetics, RNA, Messenger - metabolism, RNAi, Roles, self-compatibility, Self-incompatibility, Self-Incompatibility in Flowering Plants - physiology, Transcription, Transgenic plants