Details

Autor(en) / Beteiligte

• Clabaut, Maximilien
• Suet, Amandine
• Racine, Pierre-Jean
• Tahrioui, Ali
• Verdon, Julien
• Barreau, Magalie
• Maillot, Olivier
• Le Tirant, Agathe
• Karsybayeva, Madina
• Kremser, Coralie
• Redziniak, Gérard
• Duclairoir-Poc, Cécile
• Pichon, Chantal
• Chevalier, Sylvie
• Feuilloley, Marc G J

Titel

Effect of 17β-estradiol on a human vaginal Lactobacillus crispatus strain

Ist Teil von

• Scientific reports, 2021-03, Vol.11 (1), p.7133-7133, Article 7133

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Lactobacilli and estrogens play essential roles in vaginal homeostasis. We investigated the potential direct effect of 17β-estradiol on a vaginal strain of Lactobacillus crispatus, the major bacterial species of the vaginal microbiota. 17β-estradiol (10 to 10  M) had no effect on L. crispatus growth, but markedly affected the membrane dynamics of this bacterium. This effect appeared consistent with a signal transduction process. The surface polarity and aggregation potential of the bacterium were unaffected by exposure to 17β-estradiol, but its mean size was significantly reduced. 17β-estradiol also promoted biosurfactant production by L. crispatus and adhesion to vaginal VK2/E6E7 cells, but had little effect on bacterial biofilm formation activity. Bioinformatic analysis of L. crispatus identified a membrane lipid raft-associated stomatin/prohibitin/flotillin/HflK domain containing protein as a potential 17β-estradiol binding site. Overall, our results reveal direct effects of 17β-estradiol on L. crispatus. These effects are of potential importance in the physiology of the vaginal environment, through the promotion of lactobacillus adhesion to the mucosa and protection against pathogens.