Nature communications, 2019-02, Vol.10 (1), p.663-663, Article 663
2019
Details
- Autor(en) / Beteiligte
- Titel
- The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer
- Ist Teil von
- Nature communications, 2019-02, Vol.10 (1), p.663-663, Article 663
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- The biological role of miR-500a-5p has not yet been reported in the context of colorectal cancer (CRC). Here, we show that miR-500a-5p expression is decreased in CRC tissues compared with adjacent normal tissues. Low miR-500a-5p expression is associated with malignant progression. Moreover, transfection of CRC cells with miR-500a-5p induces G0/G1 cell cycle arrest and inhibits their growth and migration. Mechanistically, miR-500a-5p directly targets HDAC2 and inhibits HDAC2-mediated proliferation in CRC in nude mice. Furthermore, YY1 binds to the promoter of miR-500a-5p and negatively regulates its transcription. Restoration of miR-500a-5p expression is up-regulated via the p300/YY1/HDAC2 complex. Besides, therapeutic delivery of miR-500a-5p significantly suppresses tumour development in a xenograft tumour model and a HDAC2 inhibitor FK228-treated CRC model. Our studies demonstrate that miR-500a-5p functions as a tumour suppressor in CRC by targeting the p300/YY1/HDAC2 axis, which contributes to the development of and provides new potential candidates for CRC therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-018-08225-3Titel-ID: cdi_doaj_primary_oai_doaj_org_article_32fb40eb2fb94539af63c954f7d4b1f9
- Format
- –
- Schlagworte
- Animals, Apoptosis - genetics, Apoptosis - physiology, Cancer, Cell cycle, Cell Line, Cell Line, Tumor, Cell proliferation, Cell Proliferation - genetics, Cell Proliferation - physiology, Colorectal cancer, Colorectal carcinoma, Colorectal Neoplasms - genetics, Colorectal Neoplasms - metabolism, E1A-Associated p300 Protein - genetics, E1A-Associated p300 Protein - metabolism, Event-related potentials, Female, Gene Expression Regulation, Neoplastic - genetics, Gene Expression Regulation, Neoplastic - physiology, HCT116 Cells, HDAC2 protein, Histone deacetylase, Histone Deacetylase 2 - genetics, Histone Deacetylase 2 - metabolism, Humans, In Situ Nick-End Labeling, Mice, Mice, Inbred BALB C, Mice, Nude, MicroRNAs - genetics, MicroRNAs - metabolism, Restoration, Signal Transduction - genetics, Signal Transduction - physiology, Tissues, Transcription, Transfection, Tumors, Xenografts, Xenotransplantation, YY1 protein, YY1 Transcription Factor - genetics, YY1 Transcription Factor - metabolism