Details

Autor(en) / Beteiligte

• Chen, Jian-Hua
• Vanslembrouck, Bieke
• Loconte, Valentina
• Ekman, Axel
• Cortese, Mirko
• Bartenschlager, Ralf
• McDermott, Gerry
• Larabell, Carolyn A.
• Le Gros, Mark A.
• Weinhardt, Venera

Titel

A protocol for full-rotation soft X-ray tomography of single cells

Ist Teil von

• STAR protocols, 2022-03, Vol.3 (1), p.101176, Article 101176

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021). [Display omitted] •Rapid full-rotation soft X-ray tomography of single cells•Cryopreservation of biological cells in glass capillaries•Quantitative calibration of X-ray linear absorption coefficient•Step-by-step segmentation of main cellular organelles The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes.