Details

Autor(en) / Beteiligte

• Rotini, Alice
• Tornambè, Andrea
• Cossi, Riccardo
• Iamunno, Franco
• Benvenuto, Giovanna
• Berducci, Maria T
• Maggi, Chiara
• Thaller, Maria C
• Cicero, Anna M
• Manfra, Loredana
• Migliore, Luciana

Titel

Salinity-Based Toxicity of CuO Nanoparticles, CuO-Bulk and Cu Ion to Vibrio anguillarum

Ist Teil von

• Frontiers in microbiology, 2017-10, Vol.8, p.2076-2076

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2017

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Bacteria are used in ecotoxicology for their important role in marine ecosystems and their quick, reproducible responses. Here we applied a recently proposed method to assess the ecotoxicity of nanomaterials on the ubiquitous marine bacterium , as representative of brackish and marine ecosystems. The test allows the determination of 6-h EC in a wide range of salinity, by assessing the reduction of bacteria actively replicating and forming colonies. The toxicity of copper oxide nanoparticles (CuO NPs) at different salinities (5-20-35 ‰) was evaluated. CuSO 5H O and CuO bulk were used as reference toxicants (solubility and size control, respectively). Aggregation and stability of CuO NP in final testing dispersions were characterized; Cu dissolution and the physical interactions between and CuO NPs were also investigated. All the chemical forms of copper showed a clear dose-response relationship, although their toxicity was different. The order of decreasing toxicity was: CuSO 5H O > CuO NP > CuO bulk. As expected, the size of CuO NP aggregates increased with salinity and, concurrently, their toxicity decreased. Results confirmed the intrinsic toxicity of CuO NPs, showing modest Cu dissolution and no evidence of CuO NP internalization or induction of bacterial morphological alterations. This study showed the bioassay as an effective tool for the risk assessment of nanomaterials in marine and brackish environments.