Details

Autor(en) / Beteiligte

• Whisnant, Adam W
• Jürges, Christopher S
• Hennig, Thomas
• Wyler, Emanuel
• Prusty, Bhupesh
• Rutkowski, Andrzej J
• L'hernault, Anne
• Djakovic, Lara
• Göbel, Margarete
• Döring, Kristina
• Menegatti, Jennifer
• Antrobus, Robin
• Matheson, Nicholas J
• Künzig, Florian W H
• Mastrobuoni, Guido
• Bielow, Chris
• Kempa, Stefan
• Liang, Chunguang
• Dandekar, Thomas
• Zimmer, Ralf
• Landthaler, Markus
• Grässer, Friedrich
• Lehner, Paul J
• Friedel, Caroline C
• Erhard, Florian
• Dölken, Lars

Titel

Integrative functional genomics decodes herpes simplex virus 1

Ist Teil von

• Nature communications, 2020-04, Vol.11 (1), p.2038-14, Article 2038

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identify a total of 201 transcripts and 284 ORFs including all known and 46 novel large ORFs. This includes a so far unknown ORF in the locus deleted in the FDA-approved oncolytic virus Imlygic. Multiple transcript isoforms expressed from individual gene loci explain translation of the vast majority of ORFs as well as N-terminal extensions (NTEs) and truncations. We show that NTEs with non-canonical start codons govern the subcellular protein localization and packaging of key viral regulators and structural proteins. We extend the current nomenclature to include all viral gene products and provide a genome browser that visualizes all the obtained data from whole genome to single-nucleotide resolution.