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Regulation of Hair Follicle Growth and Development by Different Alternative Spliceosomes of FGF5 in Rabbits
Ist Teil von
Genes, 2024-04, Vol.15 (4), p.409
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2024
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
This study investigated the regulatory effect of alternative spliceosomes of the fibroblast growth factor 5 (
) gene on hair follicle (HF) growth and development in rabbits. The FGF5 alternative spliceosomes (called
,
,
) were cloned. The overexpression vector and siRNA of spliceosomes were transfected into dermal papilla cells (DPCs) to analyze the regulatory effect on DPCs. The results revealed that
and
overexpression significantly decreased
mRNA expression (
< 0.01).
overexpression significantly reduced
expression (
< 0.01).
and
possibly downregulated the expression level of
mRNA (
< 0.05), and FGF5-X3 significantly downregulated the expression level of
mRNA (
< 0.01). The FGF5 alternative spliceosomes significantly downregulated the
mRNA expression level in both cases (
< 0.01).
and
significantly increased
mRNA expression (
< 0.01). All three FGF5 alternative spliceosomes inhibited DPC proliferation. In conclusion, the expression profile of HF growth and development-related genes can be regulated by FGF5 alternative spliceosomes, inhibiting the proliferation of DPCs and has an influence on the regulation of HF growth in rabbits. This study provides insights to further investigate the mechanism of HF development in rabbits via
regulation.