International journal of molecular sciences, 2021-02, Vol.22 (4), p.1610
2021
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- Autor(en) / Beteiligte
- Titel
- Diphlorethohydroxycarmalol Isolated from Ishige okamurae Exerts Vasodilatory Effects via Calcium Signaling and PI3K/Akt/eNOS Pathway
- Ist Teil von
- International journal of molecular sciences, 2021-02, Vol.22 (4), p.1610
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Nitric oxide (NO) is released by endothelial cells in the blood vessel wall to enhance vasodilation. Marine polyphenols are known to have protective effects against vascular dysfunction and hypertension. The present study is the first to investigate how diphlorethohydroxycarmalol (DPHC) isolated from affects calcium levels, resulting in enhanced vasodilation. We examined calcium modulation with the well-known receptors, acetylcholine receptor (AchR) and vascular endothelial growth factor 2 (VEGFR2), which are related to NO formation, and further confirmed the vasodilatory effect of DPHC. We confirmed that DPHC stimulated NO production by increasing calcium levels and endothelial nitric oxide synthase (eNOS) expression. DPHC affected AchR and VEGFR2 expression, thereby influencing transient calcium intake. Specific antagonists, atropine and SU5416, were used to verify our findings. Furthermore, based on the results of in vivo experiments, we treated transgenic zebrafish with DPHC to confirm its vasodilatory effect. In conclusion, the present study showed that DPHC modulated calcium transit through AchR and VEGFR2, increasing endothelial-dependent NO production. Thus, DPHC, a natural marine component, can efficiently ameliorate cardiovascular diseases by improving vascular function.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms22041610Titel-ID: cdi_doaj_primary_oai_doaj_org_article_27cbbbc25a0548968ea212a6b80c94d2
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, Acetylcholine receptor, Acetylcholine receptors, AKT protein, Animals, Animals, Genetically Modified, Atropine, Blood vessels, Calcium, Calcium antagonists, Calcium Signaling - drug effects, Calcium signalling, Cardiovascular disease, Cardiovascular diseases, Cell Line, Cytotoxicity, Diphlorethohydroxycarmalol, Endothelial cells, Endothelial Cells - drug effects, Endothelial Cells - metabolism, Endothelium, Experiments, Growth factors, Heterocyclic Compounds, 3-Ring - isolation & purification, Heterocyclic Compounds, 3-Ring - pharmacology, Homeostasis, Humans, Hypertension, Kinases, Microscopy, Nitric oxide, Nitric Oxide - biosynthesis, Nitric Oxide Synthase Type III - metabolism, Nitric-oxide synthase, NO production, Phaeophyceae - chemistry, Phosphatidylinositol 3-Kinases - metabolism, Phosphorylation, Polyphenols, Protein expression, Proteins, Proto-Oncogene Proteins c-akt - metabolism, Receptors, Cholinergic - metabolism, Signal Transduction - drug effects, Vascular endothelial growth factor, Vascular endothelial growth factor receptor 2, Vascular Endothelial Growth Factor Receptor-2 - metabolism, Vasodilation, Vasodilation - drug effects, Vasodilator Agents - isolation & purification, Vasodilator Agents - pharmacology, VEGFR2, Zebrafish