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Complete sequence and comparative analysis of the mitochondrial genome of the rare and endangered Clematis acerifolia , the first clematis mitogenome to provide new insights into the phylogenetic evolutionary status of the genus
Ist Teil von
Frontiers in genetics, 2023-01, Vol.13, p.1050040-1050040
Ort / Verlag
Switzerland: Frontiers Media S.A
Erscheinungsjahr
2023
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
is one of the large worldwide genera of the Ranunculaceae Juss. Family, with high ornamental and medicinal value. China is the modern distribution centre of
with abundant natural populations. Due to the complexity and high morphological diversity of
, the genus is difficult to classify systematically, and in particular, the phylogenetic position of the endangered
is highly controversial. The use of the mitochondrial complete genome is a powerful molecular method that is frequently used for inferring plants phylogenies. However, studies on
mitogenome are rare, thus limiting our full understanding of its phylogeny and genome evolution. Here, we sequenced and annotated the
mt genome using Illumina short- and Nanopore long-reads, characterized the species first complete mitogenome, and performed a comparative phylogenetic analysis with its close relatives. The total length of the
mitogenome is 698,247 bp and the main structure is multi-branched (linear molecule 1 and circular molecule 2). We annotated 55 genes, including 35 protein-coding, 17 tRNA, and 3 rRNA genes. The
mitogenome has extremely unconserved structurally, with extensive sequence transfer between the chloroplast and mitochondrial organelles, sequence repeats, and RNA editing. The phylogenetic position of
was determined by constructing the species mitogenome with 24 angiosperms. Further, our
mitogenome characteristics investigation included GC contents, codon usage, repeats and synteny analysis. Overall, our results are expected to provide fundamental information for
mitogenome evolution and confirm the validity of mitochondrial analysis in determining the phylogenetic positioning of
plants.