Details

Autor(en) / Beteiligte

• Jeon, Eunyoung
• Koo, Bonhan
• Kim, Suyeon
• Kim, Jieun
• Yu, Yeonuk
• Jang, Hyowon
• Lee, Minju
• Kim, Sung-Han
• Kang, Taejoon
• Kim, Sang Kyung
• Kwak, Rhokyun
• Shin, Yong
• Lee, Joonseok

Titel

Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Ist Teil von

• Nature communications, 2024-02, Vol.15 (1), p.1366-1366, Article 1366

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.