Details

Autor(en) / Beteiligte

• Rosenberg, Yvonne J
• Ordonez, Tracy
• Khanwalkar, Urjeet S
• Barnette, Philip
• Pandey, Shilpi
• Backes, Iara M
• Otero, Claire E
• Goldberg, Benjamin S
• Crowley, Andrew R
• Leib, David A
• Shapiro, Mariya B
• Jiang, Xiaoming
• Urban, Lori A
• Lees, Jonathan
• Hessell, Ann J
• Permar, Sallie
• Haigwood, Nancy L
• Ackerman, Margaret E
• Swanstrom, Ronald

Titel

Evidence for the Role of a Second Fc-Binding Receptor in Placental IgG Transfer in Nonhuman Primates

Ist Teil von

• mBio, 2023-04, Vol.14 (2), p.e0034123-e0034123

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Transplacental transfer of maternal antibodies provides the fetus and newborn with passive protection against infectious diseases. While the role of the highly conserved neonatal Fc receptor (FcRn) in transfer of IgG in mammals is undisputed, recent reports have suggested that a second receptor may contribute to transport in humans. We report poor transfer efficiency of plant-expressed recombinant HIV-specific antibodies, including engineered variants with high FcRn affinity, following subcutaneous infusion into rhesus macaques close to parturition. Unexpectedly, unlike those derived from mammalian tissue culture, plant-derived antibodies were essentially unable to cross macaque placentas. This defect was associated with poor Fcγ receptor binding and altered Fc glycans and was not recapitulated in mice. These results suggest that maternal-fetal transfer of IgG across the three-layer primate placenta may require a second receptor and suggest a means of providing maternal antibody treatments during pregnancy while avoiding fetal harm. This study compared the ability of several human HIV envelope-directed monoclonal antibodies produced in plants with the same antibodies produced in mammalian cells for their ability to cross monkey and mouse placentas. We found that the two types of antibodies have comparable transfer efficiencies in mice, but they are differentially transferred across macaque placentas, consistent with a two-receptor IgG transport model in primates. Importantly, plant-produced monoclonal antibodies have excellent binding characteristics for human FcRn receptors, permitting desirable pharmacokinetics in humans. The lack of efficient transfer across the primate placenta suggests that therapeutic plant-based antibody treatments against autoimmune diseases and cancer could be provided to the mother while avoiding transfer and preventing harm to the fetus.