International journal of molecular sciences, 2019-11, Vol.20 (21), p.5468
2019
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- Autor(en) / Beteiligte
- Titel
- Biodistribution of Mesenchymal Stem Cell-Derived Extracellular Vesicles in a Radiation Injury Bone Marrow Murine Model
- Ist Teil von
- International journal of molecular sciences, 2019-11, Vol.20 (21), p.5468
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We have previously shown that injury induced by irradiation to murine marrow can be partially or completely reversed by exposure to human or murine mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). Investigation of the biodistribution of EVs in vivo is essential for understanding EV biology. In this study, we evaluated the DiD lipid dye labeled MSC-EV biodistribution in mice under different conditions, including different MSC-EV doses and injection schedules, time post MSC-EV injection, and doses of radiation. DiD-labeled MSC-EVs appeared highest in the liver and spleen; lower in bone marrow of the tibia, femur, and spine; and were undetectable in the heart, kidney and lung, while a predominant EV accumulation was detected in the lung of mice infused with human lung fibroblast cell derived EVs. There was significantly increased MSC-EV accumulation in the spleen and bone marrow (tibia and femur) post radiation appearing with an increase of MSC-EV uptake by CD11b+ and F4/80+ cells, but not by B220 cells, compared to those organs from non-irradiated mice. We further demonstrated that increasing levels of irradiation caused a selective increase in vesicle homing to marrow. This accumulation of MSC-EVs at the site of injured bone marrow could be detected as early as 1 h after MSC- EV injection and was not significantly different between 2 and 24 h post MSC-EV injection. Our study indicates that irradiation damage to hematopoietic tissue in the spleen and marrow targets MSC-EVs to these tissues.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms20215468Titel-ID: cdi_doaj_primary_oai_doaj_org_article_25eb97977e2d4845adf2ef3a45fa37e9
- Format
- –
- Schlagworte
- Accumulation, Animal models, Animals, Biodistribution, Bone marrow, Bone Marrow - metabolism, Bone Marrow - pathology, Bone Marrow - radiation effects, CD11b antigen, Cells, Cultured, Coloring Agents - chemistry, extracellular vesicle, Extracellular vesicles, Extracellular Vesicles - chemistry, Extracellular Vesicles - metabolism, Extracellular Vesicles - transplantation, Femur, Humans, In vivo methods and tests, Injection, Kidneys, Lipids, Liver - metabolism, Lungs, Male, mesenchymal stem cell, Mesenchymal stem cells, Mesenchymal Stem Cells - chemistry, Mesenchymal Stem Cells - metabolism, Mice, Inbred C57BL, Microscopy, Microscopy, Confocal, Microscopy, Fluorescence, Organs, Protein expression, Radiation, Radiation damage, Radiation injuries, Radiation Injuries - metabolism, radiation injury, Schedules, Spleen, Spleen - metabolism, Stem cells, Tibia, Tomography, Vesicles