Details

Autor(en) / Beteiligte

• Sessa, Raffaele
• Trombetti, Silvia
• Bianco, Alessandra Lo
• Amendola, Giovanni
• Catapano, Rosa
• Cesaro, Elena
• Petruzziello, Fara
• D'Armiento, Maria
• Maruotti, Giuseppe Maria
• Menna, Giuseppe
• Izzo, Paola
• Grosso, Michela

Titel

miR-1202 acts as anti-oncomiR in myeloid leukaemia by down-modulating GATA-1S expression

Ist Teil von

• Open biology, 2024-02, Vol.14 (2), p.1-17

Ort / Verlag

London: The Royal Society Publishing

Erscheinungsjahr

2024

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Transient abnormal myelopoiesis (TAM) is a Down syndrome-related preleukaemic condition characterized by somatic mutations in the haematopoietic transcription factor GATA-l that result in exclusive production of its shorter isoform (GATA-1s). Given the common hallmark of altered miRNA expression profiles in haematological malignancies and the proleukaemic role of GATA-1s, we aimed to search for miRNAs potentially able to modulate the expression of GATA-1 isoforms. Starting from an in silico prediction of miRNA binding sites in the GATA-1 transcript, miR-1202 came into our sight as potential regulator of GATA-1 expression. Expression studies in K562 cells revealed that miR-1202 directly targets GATA-1, negatively regulates its expression, impairs GATA-1s production, reduces cell proliferation, and increases apoptosis sensitivity. Furthermore, data from TAM and myeloid leukaemia patients provided substantial support to our study by showing that miR-1202 down-modulation is accompanied by increased GATA-1 levels, with more marked effects on GATA-1s. These findings indicate that miR-1202 acts as an anti-oncomiR in myeloid cells and may impact leukaemogenesis at least in part by down-modulating GATA-1s levels.