Details

Autor(en) / Beteiligte

• Simonis, Nicolas
• Rual, Jean-François
• Lemmens, Irma
• Boxus, Mathieu
• Hirozane-Kishikawa, Tomoko
• Gatot, Jean-Stéphane
• Dricot, Amélie
• Hao, Tong
• Vertommen, Didier
• Legros, Sébastien
• Daakour, Sarah
• Klitgord, Niels
• Martin, Maud
• Willaert, Jean-François
• Dequiedt, Franck
• Navratil, Vincent
• Cusick, Michael E
• Burny, Arsène
• Van Lint, Carine
• Hill, David E
• Tavernier, Jan
• Kettmann, Richard
• Vidal, Marc
• Twizere, Jean-Claude

Titel

Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses

Ist Teil von

• Retrovirology, 2012-03, Vol.9 (1), p.26-26, Article 26

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.