Molecules (Basel, Switzerland), 2018-11, Vol.23 (11), p.3040
2018
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- Autor(en) / Beteiligte
- Titel
- In Vitro Evaluation of Sulforaphane and a Natural Analog as Potent Inducers of 5-Fluorouracil Anticancer Activity
- Ist Teil von
- Molecules (Basel, Switzerland), 2018-11, Vol.23 (11), p.3040
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Isothiocyanates (R-NCS) are sulphur-containing phytochemicals. The main source are plants of the family. The best known plant-derived isothiocyanate is sulforaphane that has exhibited anticancer activity in both in vivo and in vitro studies. Recent attempts to expand their use in cancer therapy involve combining them with standard chemotherapeutics in order to increase their therapeutic efficacy. The aim of this paper is to determine the impact of sulforaphane and its natural analog alyssin on the anticancer activity of the well-known anticancer drug 5-fluorouracil. The type of drug-drug interactions was determined in prostate and colon cancer cell lines. Confocal microscopy, western blot and flow cytometry methods were employed to determine the mechanism of cytotoxic and cytostatic action of the combinations. The study revealed that additive or synergistic interactions were observed between 5-fluorouracil and both isothiocyanates, which enhanced the anticancer activity of 5-fluorouracil, particularly in colon cancer cell lines. An increased cytostatic effect was observed in case of alyssin while for sulforaphane the synergistic interaction with 5-fluorouracil involved an intensification of apoptotic cell death.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules23113040Titel-ID: cdi_doaj_primary_oai_doaj_org_article_24bea7ab970d4efb92719fd5ec32ef1b
- Format
- –
- Schlagworte
- 5-Fluorouracil, Antineoplastic Agents - pharmacology, Antitumor activity, Apoptosis, Caco-2 Cells, Cancer therapies, Caspases - metabolism, Cell cycle, Cell death, Cell growth, Cell Line, Tumor, Cell Proliferation - drug effects, Cell Survival - drug effects, Colon, Colon cancer, Colorectal cancer, Confocal microscopy, Cytostatic Agents - pharmacology, Cytotoxicity, drug interactions, Drug Synergism, Drugs, Enzymes, Flow cytometry, Fluorouracil - pharmacology, Gene Expression Regulation, Neoplastic - drug effects, HT29 Cells, Humans, In Vitro Techniques, In vivo methods and tests, Isothiocyanate, Isothiocyanates - pharmacology, Neoplasms - drug therapy, Neoplasms - metabolism, Plants, Prostate cancer, Signal Transduction - drug effects, Sulforaphane, Sulfur, Thiocyanates - pharmacology, Toxicity, Tumor cell lines, Tumors