Details

Autor(en) / Beteiligte

• Gou, Yarun
• Sun, Jian
• Liu, Jun
• Chen, Hong
• Kan, Juan
• Qian, Chunlu
• Zhang, Nianfeng
• Jin, Changhai

Titel

Structural characterization of a water-soluble purple sweet potato polysaccharide and its effect on intestinal inflammation in mice

Ist Teil von

• Journal of functional foods, 2019-10, Vol.61, p.103502, Article 103502

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •A water-soluble polysaccharide (WPSPP-1) was isolated from purple sweet potato.•WPSPP-1 was mainly composed of →4)-α-D-Glcp-(1→ and →4,6)-α-D-Glcp-(1→.•WPSPP-1 could improve inflammatory lesions in vivo.•WPSPP-1 could adjust microbial diversity caused by intestinal inflammation. A water-soluble polysaccharide (WPSPP-1) was isolated from purple sweet potato. The chemical structures of WPSPP-1 were characterized by high performance size-exclusion chromatography, methylation analysis, and 1D (1H, 13C) and 2D NMR spectra. Results showed the molecular weight of WPSPP-1 were 103 kDa. Monosaccharide analysis revealed WPSPP-1 consisted of glucose and mannose in the molar ratio of 20.44:1.00. The backbones of WPSPP-1 were composed of 1,6-α-D-Glcp, 1,4-α-D-Glcp, 1,2-α-D-Manp and 1,4,6-α-D-Glcp with β-D-Glcp on the branches. In vivo anti-inflammatory effect of WPSPP-1 on dextran sulfate sodium (DSS)-induced inflammatory mice was also determined. Results indicate that WPSPP-1 could improve inflammatory lesions by increasing IL-10, SOD and T-AOC levels and decreasing IL-1β, TNF-α, IL-6 and MDA levels. In addition, the consumption of WPSPP-1 adjusted the reduction of microbial diversity caused by intestinal inflammation, and restored the microbiota of the enteritis model group close to normal level.