Details

Autor(en) / Beteiligte

• Shin, Jong Cheol
• Jeong, Jeong-Yeon
• Son, Seon Gyu
• Choi, Sang-Haeng
• Nam, Ho-Chul
• Yoon, Tae-Ho
• Kim, Hyo-Jun
• Choi, Dong-Geun
• Lee, Hwarang
• Lee, Ukyeol
• Yang, Seon-Mo
• Kang, Il
• Jung, Dae-Young
• Lee, Han Woo
• Lee, Moon-Keun
• Lee, Tae Jae
• Kim, Geehong
• Park, Han-Oh
• Lee, Sung-Woon

Titel

Developing centrifugal force real-time digital PCR for detecting extremely low DNA concentration

Ist Teil von

• Scientific reports, 2024-05, Vol.14 (1), p.11522-11522, Article 11522

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Digital PCR (dPCR) is a technique for absolute quantification of nucleic acid molecules. To develop a dPCR technique that enables more accurate nucleic acid detection and quantification, we established a novel dPCR apparatus known as centrifugal force real-time dPCR (crdPCR). This system is efficient than other systems with only 2.14% liquid loss by dispensing samples using centrifugal force. Moreover, we applied a technique for analyzing the real-time graph of the each micro-wells and distinguishing true/false positives using artificial intelligence to mitigate the rain, a persistent issue with dPCR. The limits of detection and quantification were 1.38 and 4.19 copies/μL, respectively, showing a two-fold higher sensitivity than that of other comparable devices. With the integration of this new technology, crdPCR will significantly contribute to research on next-generation PCR targeting absolute micro-analysis.