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BibTeX
Turnip Mosaic Virus Transcriptional Slippage Dynamics and Distribution in RNA Subpopulations
Molecular plant-microbe interactions, 2022-09, Vol.35 (9), p.835-844
Kärblane, Kairi
Firth, Andrew E
Olspert, Allan
2022
Details
Autor(en) / Beteiligte
Kärblane, Kairi
Firth, Andrew E
Olspert, Allan
Titel
Turnip Mosaic Virus Transcriptional Slippage Dynamics and Distribution in RNA Subpopulations
Ist Teil von
Molecular plant-microbe interactions, 2022-09, Vol.35 (9), p.835-844
Ort / Verlag
United States: American Phytopathological Society
Erscheinungsjahr
2022
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Potyviruses comprise the largest and most important group of plant positive-strand RNA viruses. The potyviral cell-to-cell movement protein P3N-PIPO is expressed via transcriptional slippage at a conserved GAAAAAA sequence, leading to insertion of an extra 'A' in a proportion of viral transcripts. Transcriptional slippage is determined by the potyviral replicase, the conserved slippery site, and its flanking nucleotides. Here, we investigate the dynamics of transcriptional slippage at different slip-site sequences, infection stages, and environmental conditions. We detect a modest increase in the level of transcripts with insertion towards later timepoints. In addition, we investigate the fate of transcripts with insertion by separately looking at different RNA subpopulations: (+)RNA, (-)RNA, translated RNA, and virion RNA. We find differences in insertional slippage between (+)RNA and (-)RNA but not other subpopulations. Our results suggest that there can be selection against the use of (-)RNAs with insertions as templates for transcription or replication and demonstrate that insertional slippage can occur at high frequency also during (-)RNA synthesis. Since transcripts with insertions are potential targets for degradation, we investigate the connection to nonsense-mediated decay (NMD). We find that these transcripts are targeted to NMD, but we only observe an impact on the level of transcripts with insertion when the insertional slippage rate is high. Together, these results further our understanding of the mechanism and elucidate the dynamics of potyviral transcriptional slippage. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
Sprache
Englisch; Chinesisch
Identifikatoren
ISSN: 0894-0282
eISSN: 1943-7706
DOI: 10.1094/MPMI-03-22-0060-R
Titel-ID: cdi_doaj_primary_oai_doaj_org_article_235f3a2feb284c94ba827a4a6beefece
Format
–
Schlagworte
Conserved sequence
,
Environmental conditions
,
Insertion
,
Movement protein
,
Nicotiana - genetics
,
NMD
,
Nucleotides
,
Nucleotides - metabolism
,
PIPO
,
PISPO
,
Plant virus diseases
,
Plant viruses
,
potyvirus
,
Potyvirus - genetics
,
Potyvirus - metabolism
,
Replicase
,
RNA viruses
,
RNA, Viral - genetics
,
RNA, Viral - metabolism
,
Slippage
,
Subpopulations
,
Transcription
,
transcriptional slippage
,
Viral Proteins - genetics
,
Viral Proteins - metabolism
,
Virions
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