Details

Autor(en) / Beteiligte

• Rogojina, Anna
• Klesse, Laura J.
• Butler, Erin
• Kim, Jiwoong
• Zhang, He
• Xiao, Xue
• Guo, Lei
• Zhou, Qinbo
• Hartshorne, Taylor
• Garcia, Dawn
• Weldon, Korri
• Holland, Trevor
• Bandyopadhyay, Abhik
• Prado, Luz Perez
• Wang, Shidan
• Yang, Donghan M.
• Langevan, Anne-Marie
• Zou, Yi
• Grimes, Allison C.
• Assanasen, Chatchawin
• Gidvani-Diaz, Vinod
• Zheng, Siyuan
• Lai, Zhao
• Chen, Yidong
• Xie, Yang
• Tomlinson, Gail E.
• Skapek, Stephen X.
• Kurmasheva, Raushan T.
• Houghton, Peter J.
• Xu, Lin

Titel

Comprehensive characterization of patient-derived xenograft models of pediatric leukemia

Ist Teil von

• iScience, 2023-11, Vol.26 (11), p.108171-108171, Article 108171

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Patient-derived xenografts (PDX) remain valuable models for understanding the biology and for developing novel therapeutics. To expand current PDX models of childhood leukemia, we have developed new PDX models from Hispanic patients, a subgroup with a poorer overall outcome. Of 117 primary leukemia samples obtained, successful engraftment and serial passage in mice were achieved in 82 samples (70%). Hispanic patient samples engrafted at a rate (51/73, 70%) that was similar to non-Hispanic patient samples (31/45, 70%). With a new algorithm to remove mouse contamination in multi-omics datasets including methylation data, we found PDX models faithfully reflected somatic mutations, copy-number alterations, RNA expression, gene fusions, whole-genome methylation patterns, and immunophenotypes found in primary tumor (PT) samples in the first 50 reported here. This cohort of characterized PDX childhood leukemias represents a valuable resource in that germline DNA sequencing has allowed the unambiguous determination of somatic mutations in both PT and PDX. [Display omitted] •Patient-derived xenografts (PDXs) are important preclinical models•50 pediatric leukemia PDXs are largely derived from Hispanic ethnicity•Paired patient tumor, PDX, and germline for sequencing analysis•This cohort uncovers genomic, transcriptomic, and epigenomic features Cancer; Omics; Transcriptomics