Details

Autor(en) / Beteiligte

• Wang, Hui
• Lu, Hua
• Lei, Ying-shou
• Gong, Chen-yu
• Chen, Zhao
• Luan, Ying-qiao
• Li, Qiang
• Jian, Ying-zhen
• Wang, Hao-zheng
• Wu, Feng-lin
• Tao, Chang-li
• Shen, Han
• Bo, Hua-ben
• Shao, Hong-wei
• Zhang, Wen-feng

Titel

Development of a Self-Restricting CRISPR-Cas9 System to Reduce Off-Target Effects

Ist Teil von

• Molecular therapy. Methods & clinical development, 2020-09, Vol.18, p.390-401

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2020

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Development of the CRISPR-Cas9 gene-editing system has given rise to a new era of gene editing with wide applications in biology, medicine, agriculture, and other fields. However, the overexpression of Cas9 nuclease causes off-target effects and may trigger an immune response in vivo. Therefore, we constructed a self-restricting CRISPR-Cas9 system, where the target gene sequence corresponding to the guide RNA (gRNA) is inserted on either end of the Cas9 promoter. When double-strand breaks (DSBs) are induced in the target gene sequence, the Cas9 promoter is cut off and transcription ceases. With this system, expression of Cas9 protein at 60 h after transfection is only 10% that of the wild-type system, with about 70% promoter deletion efficiency. The target site editing efficiency and homologous recombination efficiency of the self-restricting system remain at about 50% and 30%, respectively, while the frequency of off-target indel formation decreased by 76.7%. Further, the number of indel types was also reduced from 13 to 2. Because this system does not include additional gRNA sequences, the possibility of introducing new off-target mutations is decreased. Importantly, this system is composed of a single plasmid, which could potentially be easily introduced in vivo using a viral vector or nanoparticles. [Display omitted]