Details

Autor(en) / Beteiligte

• Lin, Da-Wei
• Liu, Yang
• Lee, Yue-Qi
• Yang, Po-Jiun
• Ho, Chia-Tse
• Hong, Jui-Chung
• Hsiao, Jye-Chian
• Liao, Der-Chien
• Liang, An-Jou
• Hung, Tzu-Chiao
• Chen, Yu-Chuan
• Tu, Hsiung-Lin
• Hsu, Chao-Ping
• Huang, Hsiao-Chun

Titel

Construction of intracellular asymmetry and asymmetric division in Escherichia coli

Ist Teil von

• Nature communications, 2021-02, Vol.12 (1), p.888-888, Article 888

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• The design principle of establishing an intracellular protein gradient for asymmetric cell division is a long-standing fundamental question. While the major molecular players and their interactions have been elucidated via genetic approaches, the diversity and redundancy of natural systems complicate the extraction of critical underlying features. Here, we take a synthetic cell biology approach to construct intracellular asymmetry and asymmetric division in Escherichia coli, in which division is normally symmetric. We demonstrate that the oligomeric PopZ from Caulobacter crescentus can serve as a robust polarized scaffold to functionalize RNA polymerase. Furthermore, by using another oligomeric pole-targeting DivIVA from Bacillus subtilis, the newly synthesized protein can be constrained to further establish intracellular asymmetry, leading to asymmetric division and differentiation. Our findings suggest that the coupled oligomerization and restriction in diffusion may be a strategy for generating a spatial gradient for asymmetric cell division.