Details

Autor(en) / Beteiligte

• Deng, Lishuang
• Yin, Yue
• Xu, Zhiwen
• Li, Fengqin
• Zhao, Jun
• Deng, Huidan
• Jian, Zhijie
• Lai, Siyuan
• Sun, Xiangang
• Zhu, Ling

Titel

Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro

Ist Teil von

• Molecules (Basel, Switzerland), 2022-07, Vol.27 (14), p.4575

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Interferons (IFNs) play a major role in the host's antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to genus of the family; the infection is a global epidemic disease and a major threat to the pig industry. In this study, we found that IFN-λ3 inhibited the replication of PoRV in both MA104 cells and IPEC-J2 cells, and this inhibition was dose-dependent. Furthermore, the antiviral activity of IFN-λ3 was more potent in IPEC-J2 cells than in MA104 cells. Further research showed that IFN-λ3 and IFN-α might inhibit PoRV infection by activating ISGs, i.e., MxA, OASL and ISG15, in IPEC-J2 cells. However, the co-treatment of IFN-λ3 and IFN-α did not enhance the antiviral activity. Our data demonstrated that IFN-λ3 had antiviral activity against PoRV and may serve as a useful antiviral candidate against PoRV, as well as other viruses in swine.