Nature communications, 2021-04, Vol.12 (1), p.2385-13, Article 2385
2021
Details
- Autor(en) / Beteiligte
- Titel
- Quantitative imaging of intracellular nanoparticle exposure enables prediction of nanotherapeutic efficacy
- Ist Teil von
- Nature communications, 2021-04, Vol.12 (1), p.2385-13, Article 2385
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Nanoparticle internalisation is crucial for the precise delivery of drug/genes to its intracellular targets. Conventional quantification strategies can provide the overall profiling of nanoparticle biodistribution, but fail to unambiguously differentiate the intracellularly bioavailable particles from those in tumour intravascular and extracellular microenvironment. Herein, we develop a binary ratiometric nanoreporter (BiRN) that can specifically convert subtle pH variations involved in the endocytic events into digitised signal output, enabling the accurately quantifying of cellular internalisation without introducing extracellular contributions. Using BiRN technology, we find only 10.7-28.2% of accumulated nanoparticles are internalised into intracellular compartments with high heterogeneity within and between different tumour types. We demonstrate the therapeutic responses of nanomedicines are successfully predicted based on intracellular nanoparticle exposure rather than the overall accumulation in tumour mass. This nonlinear optical nanotechnology offers a valuable imaging tool to evaluate the tumour targeting of new nanomedicines and stratify patients for personalised cancer therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-22678-zTitel-ID: cdi_doaj_primary_oai_doaj_org_article_1e83db7f66c14a24885a735e9e8fd609
- Format
- –
- Schlagworte
- Animals, Antineoplastic Agents - administration & dosage, Bioavailability, Cell Line, Tumor - transplantation, Disease Models, Animal, Doxorubicin - administration & dosage, Drug Carriers - administration & dosage, Drug Carriers - analysis, Drug Carriers - chemistry, Exposure, Female, Fluorescent Dyes - chemistry, Heterogeneity, Humans, Intracellular, Intravital Microscopy, Medical imaging, Mice, Microenvironments, Molecular Imaging - methods, Molecular Probes - administration & dosage, Molecular Probes - analysis, Molecular Probes - chemistry, Nanoparticles, Nanoparticles - administration & dosage, Nanoparticles - analysis, Nanoparticles - chemistry, Nanotechnology, Neoplasms - diagnostic imaging, Neoplasms - drug therapy, Neoplasms - pathology, Optical Imaging - methods, Paclitaxel - administration & dosage, Patient Selection, Reproducibility of Results, Theranostic Nanomedicine - methods, Tissue Distribution, Tumor Microenvironment - drug effects, Tumors