Details

Autor(en) / Beteiligte

• Yuan, Qiuju
• Liu, Xiaodong
• Xian, Yan-fang
• Yao, Min
• Zhang, Xie
• Huang, Pengyun
• Wu, Wutian
• Lin, Zhi-Xiu

Titel

Satellite glia activation in dorsal root ganglion contributes to mechanical allodynia after selective motor fiber injury in adult rats

Ist Teil von

• Biomedicine & pharmacotherapy, 2020-07, Vol.127, p.110187-110187, Article 110187

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2020

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• •C8VA induced bilateral mechanical but not thermal hypersensitivity in fore paws of adult rats.•C8VA induced delayed and slight spinal glial activation.•C8VA resulted in rapid and robust activation of SGCs in both ipsilateral and contralateral DRG.•Treatment with Fluorocitrate (FC) suppressed SGC activation and alleviated mechanical allodynia in C8VA rats. Increasing evidence suggests that activation of satellite glia cells (SGCs) in sensory ganglia play important roles in the development of neuropathic pain. The present study aimed to investigate the involvement of SGC activation in a novel model of motor nerve injury induced pain hypersensitivity. The neuropathic pain model was established by cervical 8 ventral root avulsion (C8VA). Glial fibrillary acidic protein (GFAP) was used as a marker of SGC activation. Unilateral C8VA resulted in mechanical allodynia, but not thermal hyperalgesia in bilateral paws. Expectedly, SGCs were robustly activated on as early as 1 day and persisted for at least 7 days in the ipsilateral and contralateral dorsal root ganglia (DRG) of C6, C7 and C8 after C8VA. Double immunofluorescence showed that almost all the activated SGCs enveloped neurofilament 200 (NF200) positive myelinated neurons in DRG. Local application of fluorocitrate (FC), a glial metabolism inhibitor, significantly decreased the number of activated SGCs and alleviated bilateral mechanical allodynia. These results suggest that SGC activation contributed to ipsilateral and mirror-image pain hypersensitivity after C8VA. Inhibition of SGC activation represented a promising therapeutic strategy for the management of neuropathic pain following brachial plexus root avulsion.