Details

Autor(en) / Beteiligte

• Jansch, Charline
• Günther, Katharina
• Waider, Jonas
• Ziegler, Georg C.
• Forero, Andrea
• Kollert, Sina
• Svirin, Evgeniy
• Pühringer, Dirk
• Kwok, Chee Keong
• Ullmann, Reinhard
• Maierhofer, Anna
• Flunkert, Julia
• Haaf, Thomas
• Edenhofer, Frank
• Lesch, Klaus-Peter

Titel

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Ist Teil von

• Stem cell research, 2018-04, Vol.28, p.136-140

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2018

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner.