Details

Autor(en) / Beteiligte

• Schyns, Joey
• Bai, Qiang
• Ruscitti, Cecilia
• Radermecker, Coraline
• De Schepper, Sebastiaan
• Chakarov, Svetoslav
• Farnir, Frédéric
• Pirottin, Dimitri
• Ginhoux, Florent
• Boeckxstaens, Guy
• Bureau, Fabrice
• Marichal, Thomas

Titel

Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung

Ist Teil von

• Nature communications, 2019-09, Vol.10 (1), p.3964-16, Article 3964

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2019

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206 and CD206 IM, as well as a discrete population of extravasating CD64 CD16.2 monocytes. CD206 IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206 IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64 CD16.2 monocytes arise from intravascular Ly-6C patrolling monocytes that enter the tissue at steady-state to become putative precursors of CD206 IM. This study expands our knowledge about the complexity of lung IM and reveals an ontogenic pathway for one IM subset, an important step for elaborating future macrophage-targeted therapies.