Details

Autor(en) / Beteiligte

• Lehner, Sebastian
• Todica, Andrei
• Brunner, Stefan
• Uebleis, Christopher
• Wang, Hao
• Wängler, Carmen
• Herbach, Nadja
• Herrler, Tanja
• Böning, Guido
• Laubender, Rüdiger Paul
• Cumming, Paul
• Schirrmacher, Ralf
• Franz, Wolfgang
• Hacker, Marcus

Titel

Temporal Changes in Phosphatidylserine Expression and Glucose Metabolism after Myocardial Infarction: An in Vivo Imaging Study in Mice

Ist Teil von

• Molecular imaging, 2012-11, Vol.11 (6), p.461-470

Ort / Verlag

Los Angeles, CA: SAGE Publications

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Positron emission tomography (PET) for in vivo monitoring of phosphatidylserine externalization and glucose metabolism can potentially provide early predictors of outcome of cardioprotective therapies after myocardial infarction. We performed serial [68Ga]annexin A5 PET (annexin-PET) and [18F]fluorodeoxyglucose PET (FDG-PET) after myocardial infarction to determine the time of peak phosphatidylserine externalization in relation to impaired glucose metabolism in infracted tissue. Annexin- and FDG-PET recordings were obtained in female (C57BL6/N) mice on days 1 to 4 after ligation of the left anterior descending (LAD) artery. [68Ga]annexin A5 uptake (%ID/g) in the LAD artery territory increased from 1.7 ± 1.1 on day 1 to 5.0 ± 3.3 on day 2 and then declined to 2.0 ± 1.4 on day 3 (p = .047 vs day 2) and 1.6 ± 1.4 on day 4 (p = .014 vs day 2). These results matched apoptosis rates as estimated by autoradiography and fluorescein staining. FDG uptake (%ID/g) declined from 28 ± 14 on day 1 to 14 ± 3.5 on day 4 (p < .0001 vs day 1). Whereas FDG-PET revealed continuous loss of cell viability after permanent LAD artery occlusion, annexin-PET indicated peak phosphatidylserine expression at day 2, which might be the optimal time point for therapy monitoring.