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Species community of invertebrates in 10 antropogenically transformed ecosystems on the territory of Tunel’na gully (south-west part of Dnipropetrovsk, Ukraine) is investigated. The plant cover of sample areas is shortly described. Taxonomic and ecomorphical structure, indices of biological diversity of litter invertebrate communities are analysed. The invertebrate species inhabited the Tunel’na gully and listed in the Red Data Book of Ukraine and Dnepropetrovsk province are described. The value of the inspected territory for the conservation of rare and endangered animal species is underlined. The creation of new nature protected reservation in the Tunel’na gully is proposed