Details

Autor(en) / Beteiligte

• Wang, Wei
• Lin, Liyuan
• Du, Yahui
• Song, Yanling
• Peng, Xiaoman
• Chen, Xing
• Yang, Chaoyong James

Titel

Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

Ist Teil von

• Nature communications, 2019-03, Vol.10 (1), p.1317-1317, Article 1317

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Currently, there are more than 200 fecal microbiota transplantation (FMT) clinical trials worldwide. However, our knowledge of this microbial therapy is still limited. Here we develop a strategy using sequential tagging with D-amino acid-based metabolic probes (STAMP) for assessing the viabilities of transplanted microbiotas. A fluorescent D-amino acid (FDAA) is first administered to donor mice to metabolically label the gut microbiotas in vivo. The labeled microbiotas are transplanted to recipient mice, which receive a second FDAA with a different color. The surviving transplants should incorporate both FDAAs and can be readily distinguished by presenting two colors simultaneously. Isolation of surviving bacteria and 16S rDNA sequencing identify several enriched genera, suggesting the importance of specific bacteria in FMT. In addition, using STAMP, we evaluate the effects on transplant survival of pre-treating recipients using different antibiotics. We propose STAMP as a versatile tool for deciphering the complex biology of FMT, and potentially improving its treatment efficacy. The mechanisms underlying the success or failure of fecal microbiota transplantation (FMT) are unclear. Here, Wang et al. use a mouse model of FMT to show that fluorescent D-amino acids can be used to track the transplanted microbiota in the recipient animals.