Details

Autor(en) / Beteiligte

• Arrojo e Drigo, Rafael
• Jacob, Stefan
• García-Prieto, Concha F.
• Zheng, Xiaofeng
• Fukuda, Masahiro
• Nhu, Hoa Tran Thi
• Stelmashenko, Olga
• Peçanha, Flavia Letícia Martins
• Rodriguez-Diaz, Rayner
• Bushong, Eric
• Deerinck, Thomas
• Phan, Sebastien
• Ali, Yusuf
• Leibiger, Ingo
• Chua, Minni
• Boudier, Thomas
• Song, Sang-Ho
• Graf, Martin
• Augustine, George J.
• Ellisman, Mark H.
• Berggren, Per-Olof

Titel

Structural basis for delta cell paracrine regulation in pancreatic islets

Ist Teil von

• Nature communications, 2019-08, Vol.10 (1), p.3700-12, Article 3700

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2019

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Little is known about the role of islet delta cells in regulating blood glucose homeostasis in vivo. Delta cells are important paracrine regulators of beta cell and alpha cell secretory activity, however the structural basis underlying this regulation has yet to be determined. Most delta cells are elongated and have a well-defined cell soma and a filopodia-like structure. Using in vivo optogenetics and high-speed Ca 2+ imaging, we show that these filopodia are dynamic structures that contain a secretory machinery, enabling the delta cell to reach a large number of beta cells within the islet. This provides for efficient regulation of beta cell activity and is modulated by endogenous IGF-1/VEGF-A signaling. In pre-diabetes, delta cells undergo morphological changes that may be a compensation to maintain paracrine regulation of the beta cell. Our data provides an integrated picture of how delta cells can modulate beta cell activity under physiological conditions. Pancreatic islets are composed of alpha-, beta-, as well as delta-cells and appropriate regulation of glucose homeostasis relies on auto- and paracrine cellular communication. Here, the authors study the role of delta-cell filopodia in this context by employing optogenetic and calcium imaging approaches.