Molecular cancer, 2017-06, Vol.16 (1), p.108-15, Article 108
2017
Details
- Autor(en) / Beteiligte
- Titel
- PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway
- Ist Teil von
- Molecular cancer, 2017-06, Vol.16 (1), p.108-15, Article 108
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- Protein regulator of cytokinesis-1 (PRC1) belongs to the microtubule-associated proteins (MAPs) family, and is involved in cytokinesis. Recent investigations suggest PRC1 involvement in human carcinogenesis, including breast carcinoma, hepatocellular carcinoma and etc. However, whether PRC1 contributes to lung adenocarcinoma tumorigenesis remains unknown. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blotting and Immunohistochemical staining (IHC) were used to evaluate and contrast the PRC1 expression profile in lung adenocarcinoma and adjacent normal lung tissues. We examined the clinical use of PRC1 in lung adenocarcinoma prognosis. Additionally, the tumorigenesis impact of PRC1 in lung adenocarcinoma cells was verified via in vitro and in vivo metastasis and tumorigenesis assays. Notably, Next Generation Sequencing (NGS) was performed to investigate the molecular mechanism underlying the oncogenic role of PRC1 in lung adenocarcinoma. PRC1 mRNA and protein expressions were upregulated in lung adenocarcinoma tissues compared to adjacent normal lung tissues. PRC1 protein overexpression correlated with lymph node metastasis and was an independent poor prognostic factor for lung adenocarcinoma patients. Our data implied that PRC1 depletion limited the proliferation and invasion of lung adenocarcinoma cells in vitro and lowered tumor development and lung metastasis in vivo. Remarkably, limiting PRC1 substantially prompted G2/M phase cell cycle arrest and apoptosis. Mechanistically, by conducting NGS on PRC1-depleted A549 cells and control cells, we discovered that PRC1 expression was significantly correlated with the Wnt signaling pathway. This investigation offers confirmation that PRC1 is a prognostic and promising therapeutic biomarker for people with lung adenocarcinoma and takes on a key part in the activation of the Wnt/β-catenin pathway in lung adenocarcinoma development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1476-4598eISSN: 1476-4598DOI: 10.1186/s12943-017-0682-zTitel-ID: cdi_doaj_primary_oai_doaj_org_article_185aaceab83f49ce94e4b8a7968be66e
- Format
- –
- Schlagworte
- Adenocarcinoma, Adenocarcinoma - genetics, Adenocarcinoma - metabolism, Adenocarcinoma - mortality, Adenocarcinoma - pathology, Adenocarcinoma of Lung, Aged, Animals, Apoptosis, Apoptosis - genetics, Assaying, Autophagy, beta Catenin - genetics, beta Catenin - metabolism, Bioindicators, Bladder cancer, Breast cancer, Breast carcinoma, Carcinogenesis, Cell cycle, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Line, Tumor, Cell proliferation, Chains (polymeric), Cytokinesis, Depletion, DNA methylation, Female, G2 Phase Cell Cycle Checkpoints - genetics, Gastric cancer, Gene expression, Gene Expression Regulation, Neoplastic, Genomes, Hepatocellular carcinoma, High-Throughput Nucleotide Sequencing, Humans, Immunoglobulins, Kinases, Liver cancer, Lung adenocarcinoma, Lung cancer, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - mortality, Lung Neoplasms - pathology, Lungs, Lymph, Lymph nodes, Male, Medical prognosis, Metastases, Metastasis, Mice, Inbred BALB C, Microtubule-associated proteins, Middle Aged, Polymerase chain reaction, PRC1, Prognosis, Proliferation, Protein expression, Proteins, Rodents, Signal transduction, Staining, Tissues, Tumorigenesis, Western blotting, Wnt protein, Wnt Signaling Pathway - physiology, Wnt/β-catenin signaling, Xenograft Model Antitumor Assays, β-Catenin