Details

Autor(en) / Beteiligte

• Willocx, Marie
• Van der Beeten, Iris
• Asselman, Pieter
• Delgat, Lynn
• Baert, Wim
• Janssens, Steven B.
• Leliaert, Frederik
• Picron, Jean-François
• Vanhee, Celine

Titel

Sorting out the plants responsible for a contamination with pyrrolizidine alkaloids in spice seeds by means of LC-MS/MS and DNA barcoding: Proof of principle with cumin and anise spice seeds

Ist Teil von

• Food chemistry. Molecular sciences, 2022-07, Vol.4, p.100070-100070, Article 100070

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Identification of contaminating plants in seed spice with DNA barcoding.•The obtained data concurs the previously obtained results with DNA metabarcoding.•Heliotropium sp. is the predominant source of phytotoxic PA/PANOs in those samples.•The presence of only 2 Heliotropium seeds/jar can render a sample non-compliant.•The benefit combining chemical and molecular approach to check for phytotoxins. High value commodities such as spices suffer from occasional contaminations of both chemical and biological origin. Consequently, quality control and safety monitoring has become a pressing issue for the spice industry. Two recent independent studies showed that at least one third of the analyzed cumin and green anise spice seeds samples surpassed the by the European Union recently established threshold value for toxic pyrrolizidine alkaloids (PAs) and their corresponding N-oxides (PANOs). These heterocyclic secondary plant metabolites are produced by a large number of different plant families. In those spice seeds, it was found by means of DNA metabarcoding, that predominant contamination was due to the presence of herbal material from the Heliotropium genus (Boraginaceae). Unfortunately, the use of this specific type of DNA-based identification remains controversial for the majority of the official instances and preference is still given to the use of more tangible classical approaches, including microscopy and chemical analysis. However, these methodologies often suffer from inherent drawbacks. Here we demonstrate that at least for spice seeds, a combinatory approach of microscopy, chemical analysis and classical DNA barcoding of the isolated contaminants using the matK and trnH-psbA loci, provides qualitative and quantitative information on the amount of plant material responsible for the contaminations and the extent of the contamination. The generated data also demonstrates that the presence of a very limited number of Heliotropium sp. seeds in a standard commercially available canister is sufficient to surpass the allowed threshold value, illustrating once more the importance of weed control.