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Salt- and Osmo-Responsive Sensor Histidine Kinases Activate the Bradyrhizobium diazoefficiens General Stress Response to Initiate Functional Symbiosis
Molecular plant-microbe interactions, 2022-07, Vol.35 (7), p.604-615
Wülser, Janine
Ernst, Chantal
Vetsch, Dominik
Emmenegger, Barbara
Michel, Anja
Lutz, Stefanie
Ahrens, Christian H
Vorholt, Julia A
Ledermann, Raphael
Fischer, Hans-Martin
2022
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Wülser, Janine
Ernst, Chantal
Vetsch, Dominik
Emmenegger, Barbara
Michel, Anja
Lutz, Stefanie
Ahrens, Christian H
Vorholt, Julia A
Ledermann, Raphael
Fischer, Hans-Martin
Titel
Salt- and Osmo-Responsive Sensor Histidine Kinases Activate the Bradyrhizobium diazoefficiens General Stress Response to Initiate Functional Symbiosis
Ist Teil von
Molecular plant-microbe interactions, 2022-07, Vol.35 (7), p.604-615
Ort / Verlag
United States: American Phytopathological Society
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
The general stress response (GSR) enables bacteria to sense and overcome a variety of environmental stresses. In alphaproteobacteria, stress-perceiving histidine kinases of the HWE and HisKA_2 families trigger a signaling cascade that leads to phosphorylation of the response regulator PhyR and, consequently, to activation of the GSR σ factor σ . In the nitrogen-fixing bacterium , PhyR and σ are crucial for tolerance against a variety of stresses under free-living conditions and also for efficient infection of its symbiotic host soybean. However, the molecular players involved in stress perception and activation of the GSR remained largely unknown. In this work, we first showed that a mutant variant of PhyR where the conserved phosphorylatable aspartate residue D194 was replaced by alanine (PhyR ) failed to complement the Δ mutant in symbiosis, confirming that PhyR acts as a response regulator. To identify the PhyR-activating kinases in the nitrogen-fixing symbiont, we constructed in-frame deletion mutants lacking single, distinct combinations, or all of the 11 predicted HWE and HisKA_2 kinases, which we named HRXXN histidine kinases HhkA through HhkK. Phenotypic analysis of the mutants and complemented derivatives identified two functionally redundant kinases, HhkA and HhkE, that are required for nodulation competitiveness and during initiation of symbiosis. Using σ -activity reporter strains, we further showed that both HhkA and HhkE activate the GSR in free-living cells exposed to salt and hyperosmotic stress. In conclusion, our data suggest that HhkA and HhkE trigger GSR activation in response to osmotically stressful conditions which encounters during soybean host infection.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Sprache
Englisch
Identifikatoren
ISSN: 0894-0282
eISSN: 1943-7706
DOI: 10.1094/MPMI-02-22-0051-FI
Titel-ID: cdi_doaj_primary_oai_doaj_org_article_167b69517fe24c3fadfdb6f7412b3c16
Format
–
Schlagworte
Alanine
,
alphaproteobacteria
,
Bacteria
,
Bacterial Proteins - metabolism
,
Bradyrhizobium - genetics
,
Bradyrhizobium - metabolism
,
Bradyrhizobium diazoefficiens
,
Cellular stress response
,
Competitiveness
,
Deletion mutant
,
Environmental stress
,
Gene deletion
,
Gene Expression Regulation, Bacterial
,
general stress response
,
Glycine max - microbiology
,
Histidine
,
histidine kinase
,
Histidine Kinase - genetics
,
Infections
,
Kinases
,
Living conditions
,
multiple paralog knock-out
,
Mutants
,
Nitrogen
,
Nitrogen fixation
,
Nitrogenation
,
Nodulation
,
Phosphorylation
,
Phosphotransferases
,
Sodium Chloride
,
Soybeans
,
Stress, Physiological
,
Stresses
,
Symbiosis
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