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19F MRI-fluorescence imaging dual-modal cell tracking with partially fluorinated nanoemulsions
Ist Teil von
Frontiers in bioengineering and biotechnology, 2022-11, Vol.10, p.1049750-1049750
Ort / Verlag
Frontiers Media S.A
Erscheinungsjahr
2022
Link zum Volltext
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
As a noninvasive “hot-spot” imaging technology, fluorine-19 magnetic resonance imaging (
19
F MRI) has been extensively used in cell tracking. However, the peculiar physicochemical properties of perfluorocarbons (PFCs), the most commonly used
19
F MRI agents, sometimes cause low sensitivity, poor cell uptake, and misleading results. In this study, a partially fluorinated agent, perfluoro-
tert
-butyl benzyl ether, was used to formulate a
19
F MRI-fluorescence imaging (FLI) dual-modal nanoemulsion for cell tracking. Compared with PFCs, the partially fluorinated agent showed considerably improved physicochemical properties, such as lower density, shorter longitudinal relaxation times, and higher solubility to fluorophores, while maintaining high
19
F MRI sensitivity. After being formulated into stable, monodisperse, and paramagnetic Fe
3+
-promoted nanoemulsions, the partially fluorinated agent was used in
19
F MRI-FLI dual imaging tracking of lung cancer A549 cells and macrophages in an inflammation mouse model.