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Cytological investigation of anther development in DGMS line Shaan-GMS in Brassica napus L
Ist Teil von
Czech Journal of Genetics and Plant Breeding, 2013-01, Vol.49 (1), p.16-23
Ort / Verlag
Prague: Czech Academy of Agricultural Sciences (CAAS)
Erscheinungsjahr
2013
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
The cytological mechanism of male sterility of Shaan-GMS, a natural mutant dominant genic male sterile (DGMS) line in Brassica napus, is not well studied. Cytological observation was made on different-size buds of DGMS line 0A30A derived from Shaan-GMS line. The pollen mother cells (PMCs) of DGMS line 0A30A were degenerating at the beginning of meiosis and could not pass the anaphase I stage, with no dyads or tetrads formed, suggesting that the DNA damage checkpoint and spindle assembly checkpoint were activated in sterile anthers. During the meiosis process of sterile anthers in the sterile plants, several kinds of abnormal meiotic cells could be observed: nuclei condensed PMCs, cells with micronuclei, collapsed cells, plasmolysis cells, cells connected with nucleoplasmic bridge, and microspore analogue developed from PMCs without meiosis but enclosed by the exine wall. The results suggested Shaan-GMS to be a new type of DGMS line in B. napus.