Details

Autor(en) / Beteiligte

• Bui, Lilian N
• Iosue, Christine L
• Wykoff, Dennis D
• Mitchell, Aaron P
• Mitchell, Aaron P.

Titel

Tup1 Paralog CgTUP11 Is a Stronger Repressor of Transcription than CgTUP1 in Candida glabrata

Ist Teil von

• mSphere, 2022-04, Vol.7 (2), p.e0076521-e0076521

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• is a well-characterized repressor of transcription in Saccharomyces cerevisiae and Candida albicans and is observed as a single-copy gene. We observe that most species that experienced a whole-genome duplication outside of the genus have two copies of in the yeast clade. We focused on Candida glabrata and demonstrated that the uncharacterized homolog, C. glabrata ( ), is most like the S. cerevisiae ( ) gene through phenotypic assays and transcriptome sequencing (RNA-seq). Whereas plays a role in gene repression, it is much less repressive in standard growth media. Through RNA-seq and reverse transcription-quantitative PCR (RT-qPCR), we observed that genes associated with pathogenicity ( , , and ) are upregulated upon deletion of either paralog, and loss of both paralogs is synergistic. Loss of the corepressor mimics the loss of both paralogs, but not to the same extent as the Δ Δ mutant for these pathogenesis-related genes. In contrast, genes involved in energy metabolism ( , , and ) exhibit similar behavior (dependence on both paralogs), but deletion of is very similar to the Δ Δ mutant. Finally, some genes ( and ) appear to only be dependent on and and not . These data indicate separable and overlapping roles for the two paralogs and that other genes may function as the Cyc8 corepressor. Through a comparison by RNA-seq of Δ, it was found that homologs regulate similar genes in the two species. This work highlights that studies focused only on may miss important biological processes because of paralog loss after genome duplication. Due to a whole-genome duplication, many yeast species related to C. glabrata have two copies of the well-characterized gene, unlike most species. This work identifies roles for the paralogs in C. glabrata, highlights the importance of the uncharacterized paralog, called , and suggests that the two paralogs have both overlapping and unique functions. The paralogs likely influence pathogenicity based on mutants upregulating genes that are associated with pathogenicity.