Details

Autor(en) / Beteiligte

• Green, Kim Y.
• Kaufman, Stuart S.
• Nagata, Bianca M.
• Chaimongkol, Natthawan
• Kim, Daniel Y.
• Levenson, Eric A.
• Tin, Christine M.
• Yardley, Allison Behrle
• Johnson, Jordan A.
• Barletta, Ana Beatriz F.
• Khan, Khalid M.
• Yazigi, Nada A.
• Subramanian, Sukanya
• Moturi, Sangeetha R.
• Fishbein, Thomas M.
• Moore, Ian N.
• Sosnovtsev, Stanislav V.

Titel

Human norovirus targets enteroendocrine epithelial cells in the small intestine

Ist Teil von

• Nature communications, 2020-06, Vol.11 (1), p.2759-2759, Article 2759

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Abstract Human noroviruses are a major cause of diarrheal illness, but pathogenesis is poorly understood. Here, we investigate the cellular tropism of norovirus in specimens from four immunocompromised patients. Abundant norovirus antigen and RNA are detected throughout the small intestinal tract in jejunal and ileal tissue from one pediatric intestinal transplant recipient with severe gastroenteritis. Negative-sense viral RNA, a marker of active viral replication, is found predominantly in intestinal epithelial cells, with chromogranin A-positive enteroendocrine cells (EECs) identified as a permissive cell type in this patient. These findings are consistent with the detection of norovirus-positive EECs in the other three immunocompromised patients. Investigation of the signaling pathways induced in EECs that mediate communication between the gut and brain may clarify mechanisms of pathogenesis and lead to the development of in vitro model systems in which to evaluate norovirus vaccines and treatment.