Details

Autor(en) / Beteiligte

• Macdonald, Christian B
• Nedrud, David
• Grimes, Patrick Rockefeller
• Trinidad, Donovan
• Fraser, James S
• Coyote-Maestas, Willow

Titel

DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology

Ist Teil von

• Genome Biology, 2023-02, Vol.24 (1), p.36-36, Article 36

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2023

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Insertions and deletions (indels) enable evolution and cause disease. Due to technical challenges, indels are left out of most mutational scans, limiting our understanding of them in disease, biology, and evolution. We develop a low cost and bias method, DIMPLE, for systematically generating deletions, insertions, and missense mutations in genes, which we test on a range of targets, including Kir2.1. We use DIMPLE to study how indels impact potassium channel structure, disease, and evolution. We find deletions are most disruptive overall, beta sheets are most sensitive to indels, and flexible loops are sensitive to deletions yet tolerate insertions.