Details

Autor(en) / Beteiligte

• Gao, Ya-Nan
• Wang, Zi-Wei
• Su, Chuan-You
• Wang, Jia-Qi
• Zheng, Nan

Titel

Omics analysis revealed the intestinal toxicity induced by aflatoxin B1 and aflatoxin M1

Ist Teil von

• Ecotoxicology and environmental safety, 2024-06, Vol.278, p.116336-116336, Article 116336

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Aflatoxin B1 (AFB1), a common mycotoxin, can occur in agricultural products. As a metabolite of AFB1, aflatoxin M1 (AFM1) mainly exist in dairy products. These two mycotoxins threaten human health, although it is unclear how they affect the function of the intestinal barrier. In this study, mice were exposed to AFB1 (0.3 mg/kg body b.w.) and AFM1(3.0 mg/kg b.w.) either individually or in combination for 28 days to explore the main differentially expressed proteins (DEPs) and the associated enriched pathways. These findings were preliminarily verified by the transcriptomic and proteomic analyses in differentiated Caco-2 cells. The results revealed that AFB1 and AFM1 exposure in mice disrupted the function of the intestinal barrier, and the combined toxicity was greater than that of each toxin alone. Further proteomic analysis in mice demonstrated that the mechanisms underlying these differences could be explained as follows: (i) lipid metabolism was enriched by AFB1-induced DEPs. (ii) protein export pathway was stimulated by AFM1-induced DEPs. (iii) cell metabolic ability was inhibited (as evidenced by changes in UDP-GT1, UDP-GT2, and Gatm6), apoptosis was induced (MAP4K3), and epithelial cell integrity was disrupted (Claudin7 and IQGAP2), resulting in more extensive intestinal damage after combined treatment. In conclusion, the hazardous impact of co-exposure to AFB1 and AFM1 from proteomic perspectives was demonstrated in the present study. [Display omitted] •AFB1 and AFM1 induced disrupted intestinal barrier in vivo.•The ether lipid metabolism was the main mechanism of AFB1-induced intestinal toxicity.•The protein export pathway was the main mechanism of AFM1-induced intestinal toxicity.•The disrupted the epithelial cells integrity was shown in the combined toxins.