Details

Autor(en) / Beteiligte

• Gil, Agnieszka A
• Carrasco-López, César
• Zhu, Liyuan
• Zhao, Evan M
• Ravindran, Pavithran T
• Wilson, Maxwell Z
• Goglia, Alexander G
• Avalos, José L
• Toettcher, Jared E

Titel

Optogenetic control of protein binding using light-switchable nanobodies

Ist Teil von

• Nature communications, 2020-08, Vol.11 (1), p.4044-4044, Article 4044

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few tools confer light-switchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in cells or light-based protein purification in vitro. Here, we report the development of opto-nanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications including reversibly binding to endogenous intracellular targets, modulating signaling pathway activity, and controlling binding to purified protein targets in vitro. This work represents a step towards programmable photoswitchable regulation of a wide variety of target proteins.