BMC cardiovascular disorders, 2011-06, Vol.11 (1), p.30-30, Article 30
2011
Details
- Autor(en) / Beteiligte
- Titel
- Intimal hyperplasia in balloon dilated coronary arteries is reduced by local delivery of the NO donor, SIN-1 via a cGMP-dependent pathway
- Ist Teil von
- BMC cardiovascular disorders, 2011-06, Vol.11 (1), p.30-30, Article 30
- Ort / Verlag
- England: BioMed Central Ltd
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To elucidate the mechanism by which local delivery of 3-morpholino-sydnonimine (SIN-1) affects intimal hyperplasia after percutaneous transluminal coronary angioplasty (PTCA). Porcine coronary arteries were treated with PTCA and immediately afterwards locally treated for 5 minutes, with a selective cytosolic guanylate cyclase inhibitor, 1 H-(1,2,4)oxadiazole(4,3-alpha)quinoxaline-1-one (ODQ) + SIN-1 or only SIN-1 using a drug delivery-balloon. Arteries were angiographically depicted, morphologically evaluated and analyzed after one and eight weeks for actin, myosin and intermediate filaments (IF) and nitric oxide synthase (NOS) contents. Luminal diameter after PCI in arteries treated with SIN-1 alone and corrected for age-growth was significantly larger as compared to ODQ + SIN-1 or to controls (p < 0.01). IF/actin ratio after one week in SIN-1 treated segments was not different compared to untreated segments, but was significantly reduced compared to ODQ + SIN-1 treated vessels (p < 0.05). Expression of endothelial NADPH diaphorase activity was significantly lower in untreated segments and in SIN-1 treated segments compared to controls and SIN-1 + ODQ treated arteries (p < 0.01). Restenosis index (p < 0.01) and intimal hyperplasia (p < 0.01) were significantly reduced while the residual lumen was increased (p < 0.01) in SIN-1 segments compared to controls and ODQ + SIN-1 treated vessels. After PTCA local delivery of high concentrations of the NO donor SIN-1 for 5 minutes inhibited injury induced neointimal hyperplasia. This favorable effect was abolished by inhibition of guanylyl cyclase indicating mediation of a cyclic guanosine 3',5'-monophosphate (cGMP)-dependent pathway. The momentary events at the time of injury play crucial role in the ensuring development of intimal hyperplasia.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1471-2261eISSN: 1471-2261DOI: 10.1186/1471-2261-11-30Titel-ID: cdi_doaj_primary_oai_doaj_org_article_0c368926e8104012857367b4ba119fbb
- Format
- –
- Schlagworte
- Actins - metabolism, Analysis of Variance, Angioplasty, Angioplasty, Balloon, Coronary - adverse effects, Animals, Cardiac and Cardiovascular Systems, Care and treatment, Clinical Medicine, Coronary Angiography, Coronary Restenosis - diagnosis, Coronary Restenosis - etiology, Coronary Restenosis - metabolism, Coronary Restenosis - prevention & control, Coronary Vessels - drug effects, Coronary Vessels - metabolism, Coronary Vessels - pathology, Cyclic GMP - metabolism, Diagnosis, Electrophoresis, Polyacrylamide Gel, Endothelium-derived factors, Enzyme Inhibitors - pharmacology, Guanylate Cyclase - antagonists & inhibitors, Guanylate Cyclase - metabolism, Health aspects, Hyperplasia, Intermediate filament proteins, Intermediate Filaments - metabolism, Kardiologi, Klinisk medicin, Medical and Health Sciences, Medicin och hälsovetenskap, Molsidomine - analogs & derivatives, Molsidomine - pharmacology, Myosins - metabolism, NAD(P)H Dehydrogenase (Quinone) - metabolism, NADP - metabolism, Nitric oxide, Nitric Oxide - metabolism, Nitric Oxide Donors - pharmacology, Nitric Oxide Synthase - metabolism, Oxadiazoles - pharmacology, Physiological aspects, Quinoxalines - pharmacology, Remodeling, Restenosis, Risk factors, Signal Transduction - drug effects, Sus scrofa, Time Factors, Transluminal angioplasty, Tunica Intima - drug effects, Tunica Intima - metabolism, Tunica Intima - pathology